(Cat. No. 200705) Rabbit Anti Mouse C11D5.3 scFv Monoclonal Antibody , PE, 25 tests

449,00 €
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1A3 specifically binds to the scFv region of a B cell maturation antigen (specific mouse monoclonal antibody mAb, clone C11D5.3). B cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B lineage cells including multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

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  • Vol. per test: 1 µl
  • Clone: 1A3
  • Antibody type: Monoclonal
  • Immunogen: scFv region of mouse mAb clone C11D5.3
  • Host species: Rabbit
  • Reactivity: Mouse
  • Application: FCM
  • Storage buffer: Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide

Preparation and Storage

  • Shipped at 2–8°C. Store at 2– 8°C.
  • The monoclonal antibody was purified by Protein A.
  • The antibody was conjugated with PE under optimum conditions.

Product Notices

  • Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  • Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

FACS Protocol

  1. Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).
  2. Count the cells number and the viability, aliquot up to 2×10 5 live cells into each tube. (Note: the cell viability must be ≥ 95%.)
  3. Wash the cells once by FACS buffer.
  4. Resuspend cells in 100 µl of diluted Rabbit Anti Mouse C11D5.3 scFv Monoclonal Antibody, PE (Cat. No. 20070 5, 1:100 diluted in FACS buffer)