(Cat. No. 212302) Anti-G4S Linker Monoclonal Antibody, hlgGC, Alexa Fluor 647, 100 tests
Beschreibung
Description
This antibody reacts with cell membrane-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain. The poly-Glycine-Serine (G4S) linker is a kind of synthetic peptide linker sequence that is flexible and unstructured. It is frequently used to link the variable heavy (VH) domain and the variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that have an extracellular domain scFv for recognizing target antigens. The linker is made up of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and usually seen as either a 15-mer (G4S) or 20-mer (G4S) in scFv-based CARs and scFv fragments.
Specification
- Size: 100 tests
- Recommended Vol. per test: 1μl
- Antibody Types: Recombinant Monoclonal Antibodies
- Antibody Format: Recombinant Human Fc Chimera
- Clone: S13
- Immunogen: Synthetic 15-mer (G4S) polypeptide
- Conjugate: Alexa Fluor 647
- Excitation/Emission Max: 651/667nm
- Host species: Rabbit
- Application: Flow Cytometry
- Storage buffer: Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300
Preparation and Storage
- Store undiluted at 2-8°C.
- Avoid prolonged exposure to light.
- Avoid freeze/thaw cycle of the reagent.
- The monoclonal antibody was purified by Protein A.
- The antibody was conjugated with Alexa Fluor 647 under optimum conditions, and unincorporated dye was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa and eyes.