(Cat. No. 212302) Anti-G4S Linker Monoclonal Antibody, hlgGC, Alexa Fluor 647, 100 tests

699,00 €
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Beschreibung

Description

This antibody reacts with cell membrane-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain. The poly-Glycine-Serine (G4S) linker is a kind of synthetic peptide linker sequence that is flexible and unstructured. It is frequently used to link the variable heavy (VH) domain and the variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that have an extracellular domain scFv for recognizing target antigens. The linker is made up of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and usually seen as either a 15-mer (G4S) or 20-mer (G4S) in scFv-based CARs and scFv fragments.


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Specification


  • Size: 100 tests
  • Recommended Vol. per test: 1μl
  • Antibody Types: Recombinant Monoclonal Antibodies
  • Antibody Format: Recombinant Human Fc Chimera
  • Clone: S13
  • Immunogen: Synthetic 15-mer (G4S) polypeptide
  • Conjugate: Alexa Fluor 647
  • Excitation/Emission Max: 651/667nm
  • Host species: Rabbit
  • Application: Flow Cytometry
  • Storage buffer: Aqueous buffered solution containing protein stabilizer and ≤0.05% ProClin 300



Preparation and Storage

  • Store undiluted at 2-8°C.
  • Avoid prolonged exposure to light.
  • Avoid freeze/thaw cycle of the reagent.
  • The monoclonal antibody was purified by Protein A.
  • The antibody was conjugated with Alexa Fluor 647 under optimum conditions, and unincorporated dye was removed.


Product Notices

  • Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  • Caution: Antibody solutions containing ProClin 300 should be handled with care. Do not take internally and avoid all contact with the skin, mucosa and eyes.