(Cat. No. 500014) Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody, Biotin, 100 tests
Beschreibung
Description
The rabbit polyclonal antibody R19 specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Specification
- Host species: Rabbit
- Antibody type: Polyclonal
- Immunogen: scFv region of a CD19-specific mouse mAb clone FMC63
- Reactivity: Mouse
- Application: FCM
- Storage buffer: Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide
Preparation and Storage
Shipped at 4°C. Store at 4°C short term (1-2 weeks). Store at -20°C in small aliquots for long term storage. Avoid freeze/thaw cycle.
The polyclonal antibody was purified by Protein A.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
FACS Protocol
- Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).
- Count the cells number and the viability, aliquot up to 2×105 live cells into each tube. (Note: the cell viability must be ≥ 95%.)
- Resuspend cells in 100 µL of diluted Blocking Reagent (Cat. No. 700000, 1:100 diluted in FACS buffer) for 45 min at 4°C.
- Wash the cells once by FACS buffer.
- Resuspend cells in 100 µL of diluted Biotin Rabbit Anti-Mouse FMC63 scFv antibody (Cat. No. 500013, 1:100 diluted in FACS buffer) for 30 min at 4°C.
- Wash the cells once by FACS buffer.
- Resuspend cells in 100 µL of diluted StreptavidinPE (Cat. No. 700006, 1:200 diluted in FACS buffer) for 30 min at 4°C.
- Wash the cells 3 times by FACS buffer and resuspend the cells in 200 µL PBS per sample.
- Transfer the cells into flow tube and analyze on Flow Cytometer. Acquisition of >10, 000 events is performed.